Mulia Dila Analisa

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Yeni Maulidah Muflihah

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Dwi Indarti

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Noor Hidayat Abu Bakar

Institute of Chemistry, Academia Sinica, Taipei, Taiwan R.O.C.

DOI: https://doi.org/10.19184/icl.v5i1.60007

ABSTRACT 

Diazinon, an organophosphate pesticide used in agriculture, may leave harmful residues on vegetables. This study determined diazinon residues in green mustard (Brassica juncea L.), optimized extraction solvents, and evaluated spraying intervals before harvest using UV–Visible spectrophotometry as a simple and low-cost method. The novelty lies in developing a validated spectrophotometric approach combined with optimized hexane:acetone extraction (9:1), offering a practical alternative to chromatographic techniques. The method showed excellent linearity (r = 0.999), LOD of 0.018 ppm, LOQ of 0.060 ppm, recovery of 87–108%, and precision with 1.87% RSD. Results indicated that longer spraying intervals reduced residue levels, highlighting the method’s reliability and the importance of proper spraying management.

Keywords: diazinon, green mustard, spectrophotometry UV-Vis, validation.