Ika Oktavianawati 

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Ulfa Wulandari

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember, MI Muhammadiyah Sidorejo, Rowokangkung, Lumajang

I Nyoman Adi Winata 

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Atok Ainur Ridho

Department of Agribusiness, Faculty of Agriculture, Universitas Muhammadiyah Jember

Yohana Ivana Kedang 

Department of Chemistry, Faculty of Agriculture, Science and Health, Universitas Timor

Dina Trianggaluh Fauziah

Department of Pharmacy, Faculty of Health Sciences, Universitas dr. Soebandi Jember

Fatimah Yusro 

PT. Mangli Djaya Raya, Petung, Jember

DOI: https://doi.org/10.19184/icl.v4i1.5995

ABSTRACT 

Na-Oogst tobacco is one of the local commodities of Jember, which has been widely used as a raw material for making cigarettes. Product diversification in processing tobacco leaves is very necessary to reduce the danger caused by cigarette consumption. It is also an effort to empower and save the economy of tobacco farmers. Tobacco leaf’ essential oil could be an alternative in maintaining tobacco production. The purpose of this study is to determine the profile (yield and chemical compounds) of Na-Oogst tobacco leaf’ essential oil content in pre- and post-fermentation. Extraction of essential oils is carried out by two methods, i.e., steam distillation and maceration. The results showed that the extraction from the maceration method produced higher yields than from steam distillation, while the extract from tobacco leaves before fermentation was lower than that after fermentation. The major identified compounds found in tobacco leaves from GC-MS analysis are neophytadiene, nicotine, ledol, phytol, and solanone. Neophytadiene is suggested as a marker compound in tobacco leaf since it is present in all extracts and essential oils in significant amounts, about 23.72-67.37% of the total identified compounds. Further molecular docking of neophytadiene against the D2 dopamine receptor is also investigated in this study.

Keywords: extraction, essential oil, fermentation, tobacco.