Anak Agung Istri Ratnadewi

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember  

Linda Faiqotul Himmah

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Tri Mulyono

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember  

Wuryanti Handayani

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

NG. Krishnabudi

Department of Economics, Faculty of Economics and Business, University of Jember  

Sudarko

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

DOI: https://doi.org/10.19184/ICL.v1i1.3

ABSTRACT 

Parameters kinetic (KM, VMax, and kCat) endo-β-1,4-D-xylanase under optimum conditions with oat spelt xylan and birchwood substrate have been investigated in this study. Hydrolysis of endo-β-1,4-D-xylanase using variation of substrate concentration (b/v) ranging from 0.2 to 1.2%. Variation of incubation time is up to 20 hours with 4 hours interval at the optimum temperature of the enzyme, 40°C. The results obtained from this study were the KM of endo-β-1,4-D-xylanase for oat spelt xylan and birchwood were 4.10 mg/ml and 0.681 mg/ml, respectively. VMax values, and kCat for oat spelt xylan substrate of 0.28 mg/ml.jam and 1.7 x 10-3 s-1. While VMax, and kCat for birchwood substrate that is 0.117 mg/U/jam and 7 x 10-4 s-1. From the results of this study we found that endo-β-1,4-D-xylanase can hydrolaze substrates which have differences solubility.   

Keywords: endo -β-1,4-D-xylanase, kinetic parameters, oat spelt xylan, birchwood.